Want to know how Vertical Laminar Flow Hoods work and get some ideas on the basic principles of this machine? This article will deliver all the facts and ideas that you should have regarding this machine in a crisp format. So, stay along!
Laminar Flow Hoods are specialized hoods that offer the perfectly sterilized ambiance to perform your experiments at. In the field of science, it is imperative to maintain some safety protocols to root out the possibility of contamination, infection, or errors in any form.
Laminar Flow Hoods are one of the lab instruments that make sure that the air circulating inside the laboratory or room is free of germs, dust, or any particulate matter. They have a broad and quite a big workspace illuminated with UV and fluorescent lamps inside the chamber.
For the sake of imagination, you can imagine a roughly rectangular or square box (having considerable area), which is closed by opaque walls on the three sides and has a transparent front wall. The workspace is quite spacious, and there are filters to make sure sterilization is maintained.
Laminar Flow Hoods are of two types mainly: Vertical and Horizontal. In order to explain how these names have derived, let us know the function of the filters fitted with the Laminar Flow Hoods. There are majorly two kinds of filters that are used here. They are High-Efficiency Particulate Air or HEPA filters and carbon filters.
The latter, although present, is not likely to contribute to the purpose of sterilization separately. This is because HEPA itself is quite a stable and efficient filter itself. It has the potential of driving out particles as small as 0.3 microns.
It follows the principle of using a uniform flow of air in parallel lines to remove contaminants. The direction of the airflow can, however, vary, such as horizontally or vertically. Hence the names Vertical and Horizontal Laminar Flow Hoods .
This was the brief about the subject mentioned in the title. In the following part of the article, let us see how different features of the Vertical Laminar Flow Hoods help it work.
The General Framework:
The primary framework remains the same for all types of Laminar Flow Hoods, i.e., they have a hooded system that helps them with the filtration purpose. It is the home for many specialized filters. Any Laminar Flow Hoods are composed of several working elements, all of which work in an integrated manner.
The structure is usually made up of stainless steel with no holes or joints, i.e., it is a single unit. This is done to avoid the collection of contaminants within the system. As already mentioned in the previous segment, they are fitted with UV-C (germicidal) and Fluorescent lamps as the source of first-line sterilization and light, respectively.
The most important feature that makes it different from the other kinds is the nature of the filter. Vertical Laminar Flow Hoods have HEPA filters along with Activated Carbon filters. The former lets the air circulating inside the laboratory pass through the filter (that is situated on top of the unit) and flow directly through the front access area.
HEPA follows the exact principle of allowing air to flow in uniform and parallel lines while flowing out of the filter for both Vertical and Horizontal Laminar Flow Hoods.
This is done so that every area inside the system can be covered uniformly and to make sure no residue is left behind, which can be a possibility if you are not using the airflow evenly. However, in the case of Vertical Laminar Flow Hoods, the airflow is only directed to downward.
This is beneficial because with the gravity acting towards downward, it is easy for the air to flow likewise.
Vertical Laminar Flow Hoods are bigger as a unit compared to the Horizontal one. They require ample space to be set up properly because most of the working elements are fitted on the top of the unit, increasing the requirement of a big space.
Since it demands a spacious room to be installed, it also offers a much spacious workspace to the users. The workspace is enough to run 2-3 individual work simultaneously. This means if your equipment for the given study is large, you can easily work inside a Vertical Laminar Flow Hood.
Also, due to the use of vertically directed airflow, less turbulence can be experienced by the utensils or equipment.
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This was the complete working principle and mechanisms followed by Vertical Laminar Flow Hoods. The article also mentions the different essential parts of the unit in order to make you understand the science behind this machine. Expectantly, you must have gotten the answers to all your questions.